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KMID : 0624620210540030164
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BMB Reports
2021 Volume.54 No. 3 p.164 ~ p.169
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Na/K-ATPase beta1-subunit associates with neuronal growth regulator 1 (NEGR1) to participate in intercellular interactions
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Cheon Yeong-Mi
Yoo A-Ra
Seo Hyun-Seok
Yun Seo-Young
Lee Hyeon-Hee
Lim Hee-Ji
Kim Young-Ho
Che Lihua
Lee Soo-Jin
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Abstract
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Neuronal growth regulator 1 (NEGR1) is a GPI-anchored membrane protein that is involved in neural cell adhesion and communication. Multiple genome wide association studies have found that NEGR1 is a generic risk factor for multiple human diseases, including obesity, autism, and depression. Recently, we reported that Negr1-/- mice showed a highly increased fat mass and affective behavior. In the present study, we identified Na/K-ATPase, beta1-subunit (ATP1B1) as an NEGR1 binding partner by yeast two-hybrid screening. NEGR1 and ATP1B1 were found to form a relatively stable complex in cells, at least partially co-localizing in membrane lipid rafts. We found that NEGR1 binds with ATP1B1 at its C-terminus, away from the binding site for the alpha subunit, and may contribute to intercellular interactions. Collectively, we report ATP1B1 as a novel NEGR1-interacting protein, which may help deciphering molecular networks underlying NEGR1-associated human diseases.
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KEYWORD
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Cancer, Cell adhesion molecule, Lipid rafts, Na/K-ATPase beta1-subunit, Neuronal growth regulator 1, Yeast two-hybrid
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